The original intention of the “the Belt and Road” strategy is to promote the economic development in countries and regions along the route through technological, capital, cultural and educational exchanges. However, the flow of infrastructure and personnel is bound to pose serious challenges to the ecological environment of the countries and regions along the route. Therefore, the research on how to strengthen the education of environmental awareness of mobile personnel is of great significance for promoting the smooth implementation of “the Belt and Road” strategy. Based on the popular phenomenon of short-term overseas study tour of college students in recent years, this paper conducts the association study of the environmental education for college students before overseas study tour and “the Belt and Road” strategy based on the overall analysis of the ecological environment status of the countries and regions along “the Belt and Road”. The research results show that the pre-school environmental education has a positive impact on the smooth implementation of “the Belt and Road”. The importance analysis and education content recommendations of environmental education for college students before overseas study tour in this paper is conducive to expanding the international influence of the “green development concept” advocated by China. Meanwhile, it is of great practical significance for the smooth implementation of “the Belt and Road” strategy.