In the cultivation of the children’s cognition for the society and culture, the game-based education in the kindergarten is an important way to develop their collaboration abilities in the games and greatly enhance children’s self-confidence and imagination. In this paper, the application of collaboration in kindergarten gamebased education was studied under the background of kindergarten multicultural education. The results have shown that children can learn about the diverse cultures through cultural game-based education, and greatly enhance their collaboration abilities as a team member in the games. The collaboration cultivated in the gamebased education is closely related to the curriculum content and the teacher’s professionalism. The frequency of teaching cases varies with game behaviors, in which construction games see the most teaching cases. This paper can serve as a theoretical reference for researches on the application of collaboration ability in children’s game-based education.