In the era of knowledge economy, knowledge producers play an important role in economic development. How to promote knowledge transfer and commercialization of knowledge achievements has become an urgent problem for many universities which are major knowledge producers. This paper analyzes the behavior characteristics and interest relationship of innovation intermediaries and universities in knowledge transfer cooperation, constructs the evolutionary game model of innovation intermediaries and universities in knowledge transfer cooperation, analyzes the strategy choice, evolution track and influence factors of the two. On this basis, the paper puts forward some strategic suggestions to promote the transfer of university knowledge.