Music has a positive effect on psychological adjustment and relieve tension and this view has been supported by clinical evidence. However, there is still no in-depth analysis of whether there are differences in the regulating effect of music with different characteristics on human psychology, which leads to the fact that current therapeutic schemes using the music to relieve tension are not targeted. In this context, this paper uses the method of EEG clinical trials to explore the role of differences in music rhythm and music mode in psychological regulation. By monitoring the variance of brainwaves of the subjects and the synchronic characteristics of brainwaves in different regions, the research proves that there are great differences in the psychological regulation effect of music with different characteristics: Compared to fast-rhythm music, the effect of slow-rhythm music in psychological regulation and relaxation is better. Compared with major music, the effect of minor music in psychological regulation and relaxation is better. The above conclusions can be well applied to the treatment of psychological disorder diseases such as anxiety disorder and depression, providing a reference for the targeted design of music therapeutic schemes.