Given the growing need to enhance learning outcomes in business education, it is important to identify factors that shape business students’ attitude and their knowledge retention. The present research investigates how learning environment, teacher support and business student academic motivation together influence business student attitude and strengthen knowledge retention. This study also assesses the moderating effect of e-learning support and the mediating effect of business student attitude. Present research adopted quantitative research methodology. Data of the present study was collected from business students by using convenience sampling. Developed questionnaire was distributed among 350 business students. Questionnaire was developed using seven-point Likert scale. Response rate of the present research was 74.57%. Collected data was analysed using Smart PLS 4. Results show that business student attitude has positive effect on knowledge retention. Also, teacher support, business student academic motivation and learning environment have positive effect on business student attitude. Also, mediating effect of business student attitude is statistically supported. Results also support moderating effect of e-learning support. Present study is among few ones that highlight the moderating effect of e-learning support. Additionally, present research is among limited ones that sheds light on mediating effect of business student attitude.